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pcdna3.1_mouseST6galI
(Plasmid #178435)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 178435 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    St6gal1
  • Alt name
    ST6 Beta-Galactoside Alpha-2,6-Sialyltransferase 1
  • Species
    M. musculus (mouse)
  • Mutation
    Coding sequnce for amino acids 85-403
  • GenBank ID
    20440 NP_001239434.1
  • Entrez Gene
    St6gal1 (a.k.a. AW742324, Siat1, St6Gal-I, St6gal, St6galI)
  • Promoter CMV
  • Tag / Fusion Protein
    • 6xHIS-TEV Cleavage Site (N terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer GAATCTCTATTTCCAGGGAGGTAAGCCACAGCCCTCCTTGCAGG
  • 3′ sequencing primer GTGCTGGATATCTGCAGAATTCTCAACAGCGATTGTTCCGGAAGCC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcdna3.1_mouseST6galI was a gift from Deborah Fass (Addgene plasmid # 178435 ; http://n2t.net/addgene:178435 ; RRID:Addgene_178435)

  • For your References section:

    The disulfide catalyst QSOX1 maintains the colon mucosal barrier by regulating Golgi glycosyltransferases. Ilani T, Reznik N, Yeshaya N, Feldman T, Vilela P, Lansky Z, Javitt G, Shemesh M, Brenner O, Elkis Y, Varsano N, Jaramillo AM, Evans CM, Fass D. EMBO J. 2022 Oct 17:e111869. doi: 10.15252/embj.2022111869. 10.15252/embj.2022111869 PubMed 36245281
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