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Addgene

ELP-TEMP/pcDNA3.1(-)
(Plasmid #178434)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 178434 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA 3.1(-)
  • Backbone size w/o insert (bp) 5400
  • Total vector size (bp) 8700
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ELP-TEMP
  • Species
    Synthetic
  • Insert Size (bp)
    3300
  • GenBank ID
    LC320161
  • Tag / Fusion Protein
    • No tag

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ELP-TEMP/pcDNA3.1(-) was a gift from Takeharu Nagai (Addgene plasmid # 178434 ; http://n2t.net/addgene:178434 ; RRID:Addgene_178434)

  • For your References section:

    A highly-sensitive genetically encoded temperature indicator exploiting a temperature-responsive elastin-like polypeptide. Vu CQ, Fukushima SI, Wazawa T, Nagai T. Sci Rep. 2021 Aug 13;11(1):16519. doi: 10.1038/s41598-021-96049-5. 10.1038/s41598-021-96049-5 PubMed 34389773
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