PB CAG-F-bpA-SV40pA-F-L-bpA-SV40pA-L-td-sfGFP
(Plasmid #178432)

• Purpose: CAG tdGFP FLP/FRT cre/lox "AND" reporter piggyBac transposon (bpA SV40pA stop)
• Depositing Lab: Mario Capecchi
• Publication: piggyBac transposon plasmids (unpublished)

Backbone

• Vector backbone: Custom
• Backbone manufacturer: Hyung-song Nam, Capecchi laboratory
• Vector type: Mammalian Expression, Cre/Lox ; FLP/FRT, piggyBac

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): DH10B
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: td-sfGFP
• Species: Synthetic

Resource Information

• Supplemental Documents:
  • ** 12 PB CAG-F-bpA-1pA-F-L-bpA-1pA-L-tdGFP-delXho1.gb
• A portion of this plasmid was derived from a plasmid made by: piggyBac ITR's were synthesized from sequences in the NCBI database. CAG promoter is from pCAGGS obtained from BCCM/LMBP. bGH pA is from Neal Copeland. SV40 pA is from Clontech. sfGFP is from Sandia Biotech. WPRE is from Miguel Ramalho-Santos. Backbone is from Stratagene.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • piggyBac Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Similar to the situation with some other plasmids in this collection, the missing four nucleotides at the 5' end of the WPRE renders it unrecognizable to SnapGene, but the expression vector is functional.