AAV-DIO-TC66T-2A-EGFP-2A-oG
(Plasmid #178429)

• Purpose: Expresses TVA(TC66T), EGFP, and optimized Rabies G (oG) protein in a FLEX cassette
• Depositing Lab: Edward Callaway
• Publication: AAV plasmids to express TVA and optimized Rabies G protein (unpublished)

Backbone

• Vector backbone: pAAV
• Total vector size (bp): 7293
• Vector type: AAV, Cre/Lox ; Adeno-associated virus

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: TVA950 Glu66→Thr
• Alt name: TC66T
• Species: Quail
• Insert Size (bp): 435
• Promoter: hSyn

Cloning Information for Gene/Insert 1

• Cloning method: Unknown
• 5′ sequencing primer: WPRE Rev

Gene/Insert 2

• Gene/Insert name: Optimized G
• Alt name: oG
• Species: Synthetic
• Insert Size (bp): 1575
• Promoter: hSyn

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: hSyn For

Gene/Insert 3

• Gene/Insert name: EGFP
• Species: Aequorea victoria
• Insert Size (bp): 711
• Promoter: hSyn

Cloning Information for Gene/Insert 3

• Cloning method: Unknown
• 5′ sequencing primer: WPRE Rev

Resource Information

• Supplemental Documents:
  • AAV-DIO-TC66T-2A-EGFP-2A-oG.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry