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Addgene

pCI-T7Max-UTR1-NTerminus8xHis-T500
(Plasmid #178421)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 178421 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PCI
  • Backbone size (bp) 2800
  • Modifications to backbone
    promoter, RBS, MCS and terminator replaced. BsaI sites removed from the whole backbone.
  • Vector type
    Bacterial Expression, Synthetic Biology
  • Promoter T7Max
  • Tags / Fusion Proteins
    • 8xHis (N terminal on backbone)
    • 8xHis (N terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCI-T7Max-UTR1-NTerminus8xHis-T500 was a gift from Kate Adamala (Addgene plasmid # 178421 ; http://n2t.net/addgene:178421 ; RRID:Addgene_178421)

  • For your References section:

    T7Max transcription system. Deich C, Cash B, Sato W, Sharon J, Aufdembrink L, Gaut NJ, Heili J, Stokes K, Engelhart AE, Adamala KP. J Biol Eng. 2023 Jan 23;17(1):4. doi: 10.1186/s13036-023-00323-1. 10.1186/s13036-023-00323-1 PubMed 36691081
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