pCI-T7Max-UTR1-CTerminus8xHis-T500
(Plasmid
#178419)
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Purpose(Empty Backbone) Empty backbone. T7Max promoter with C-His Tag
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 178419 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonePCI
- Backbone size (bp) 2800
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Modifications to backbonepromoter, RBS, MCS and terminator replaced. BsaI sites removed from the whole backbone.
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Vector typeBacterial Expression, Synthetic Biology
- Promoter T7Max
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Tag
/ Fusion Protein
- 8xHis (C terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2021.10.17.464727v1 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCI-T7Max-UTR1-CTerminus8xHis-T500 was a gift from Kate Adamala (Addgene plasmid # 178419 ; http://n2t.net/addgene:178419 ; RRID:Addgene_178419) -
For your References section:
T7Max transcription system. Deich C, Cash B, Sato W, Sharon J, Aufdembrink L, Gaut NJ, Heili J, Stokes K, Engelhart AE, Adamala KP. J Biol Eng. 2023 Jan 23;17(1):4. doi: 10.1186/s13036-023-00323-1. 10.1186/s13036-023-00323-1 PubMed 36691081
Map uploaded by the depositor.
