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pCDM8-GlyCAM-1/IgG
(Plasmid #17841)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 17841 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDM8
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 4356
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Tetracycline, 100 & 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10/P3
  • Growth instructions
    E.coli containing the P3 element
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    GlyCAM-1/IgG
  • Alt name
    GlyCAM-1 Fc
  • Alt name
    GlyCAM1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    600
  • Entrez Gene
    Glycam1 (a.k.a. Sgp50)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site HindIII (unknown if destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The pIg vector (which encodes the Fc part of human IgG1)was received by us in 1994 from David L. Simmons. In 1996 he gave us permission to distribute this vector widely. This was cloned into pCDM8 with GlyCAM-1.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDM8-GlyCAM-1/IgG was a gift from Steven Rosen (Addgene plasmid # 17841 ; http://n2t.net/addgene:17841 ; RRID:Addgene_17841)

  • For your References section:

    Sulfotransferases of two specificities function in the reconstitution of high endothelial cell ligands for L-selectin. Bistrup A, Bhakta S, Lee JK, Belov YY, Gunn MD, Zuo FR, Huang CC, Kannagi R, Rosen SD, Hemmerich S. J Cell Biol. 1999 May 17. 145(4):899-910. 10.1083/jcb.145.4.899 PubMed 10330415
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