pOME0182
(Plasmid
#178403)
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PurposeExpresses KSHV K8.1 glycoprotein hyp tagged
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 178403 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDEST47
- Backbone size w/o insert (bp) 7780
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameKSHV K8.1
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SpeciesKaposi's sarcoma-associated herpesvirus
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Insert Size (bp)788
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Tag
/ Fusion Protein
- 3xFLAG (C terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer T7 promoter (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Sequences and further information can be found at https://www.med.unc.edu/orfeome/downloads/pome
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pOME0182 was a gift from Dirk Dittmer (Addgene plasmid # 178403 ; http://n2t.net/addgene:178403 ; RRID:Addgene_178403) -
For your References section:
Novel modulators of p53-signaling encoded by unknown genes of emerging viruses. Alzhanova D, Corcoran K, Bailey AG, Long K, Taft-Benz S, Graham RL, Broussard GS, Heise M, Neumann G, Halfmann P, Kawaoka Y, Baric RS, Damania B, Dittmer DP. PLoS Pathog. 2021 Jan 7;17(1):e1009033. doi: 10.1371/journal.ppat.1009033. eCollection 2021 Jan. 10.1371/journal.ppat.1009033 PubMed 33411764