Skip to main content
Addgene

pSecTag-T210A-ATX
(Plasmid #17840)

Full plasmid sequence is not available for this item.

Loading...

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 17840 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pSecTag
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5200
  • Vector type
    Mammalian Expression
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    T210A-ATX
  • Alt name
    mutant ATX
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3000
  • Entrez Gene
    ENPP2 (a.k.a. ATX, ATX-X, AUTOTAXIN, LysoPLD, NPP2, PD-IALPHA, PDNP2)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site NotI (unknown if destroyed)
  • 5′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSecTag-T210A-ATX was a gift from Steven Rosen (Addgene plasmid # 17840 ; http://n2t.net/addgene:17840 ; RRID:Addgene_17840)
  • For your References section:

    Autotaxin, an ectoenzyme that produces lysophosphatidic acid, promotes the entry of lymphocytes into secondary lymphoid organs. Kanda H, Newton R, Klein R, Morita Y, Gunn MD, Rosen SD. Nat Immunol. 2008 Apr . 9(4):415-23. 10.1038/ni1573 PubMed 18327261