pSecTag-ATX
(Plasmid #17839)

• Depositing Lab: Steven Rosen
• Publication: Kanda et al Nat Immunol. 2008 Apr . 9(4):415-23.

Backbone

• Vector backbone: pSecTag
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5200
• Vector type: Mammalian Expression
• Selectable markers: Zeocin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Autotaxin
• Alt name: ATX
• Species: H. sapiens (human)
• Insert Size (bp): 3000
• Entrez Gene: ENPP2 (a.k.a. ATX, ATX-X, AUTOTAXIN, LysoPLD, NPP2, PD-IALPHA, PDNP2)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (unknown if destroyed)
• 3′ cloning site: NotI (unknown if destroyed)
• 5′ sequencing primer: T7

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

From the depositor:

In 1998, we purchased from Invitrogen pSecTag2A and cut it with BglII and BamH1. The BglII/BamH1 fragment was replaced with a modified fragment containing 6 Histidines at the N-Terminus that was done by generating the modification by PCR, and cloning the PCR product back into BglII/BamH1.
Sometime later, the above pSecTag2A-6HIS was digested with BamH1and Not1. The ATX (amino acids 52-863) insert was genereated by PCR, digested with BamH1/EcoR1 and by excising ATX from the original colony II cDNA library with EcoR1/Not1, which yields two pieces in two stages into the vector.
Their is a stop codon before the C-Terminus Myc tag so it will not be expressed.