pCAX-mScarlet-FBP17
(Plasmid #178366)

• Purpose: Expresses FBP17 c-terminal labeled with mScarelt in mammalian cells
• Depositing Lab: Erik Dent
• Publication: Taylor et al Life Sci Alliance. 2019 Jun 3;2(3). pii: 2/3/e201800288. doi: 10.26508/lsa.201800288. Print 2019 Jun.

Backbone

• Vector backbone: pCAX
• Backbone size w/o insert (bp): 4731
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Formin Binding Protein 17
• Alt name: FBP17
• Species: H. sapiens (human)
• Insert Size (bp): 1851
• Mutation: 400 Glutamic Acid changed to Aspartic acid (403 A to C)
• Entrez Gene: FNBP1 (a.k.a. FBP17)
• Promoter: Chicken Beta Actin

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CAGCTCCTGGGCAACGTGC
• 3′ sequencing primer: CACTCGGAAGGACATATGGG

Resource Information

• Supplemental Documents:
  • 350-pcax-fbp17-mscarlet-lf.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry