pN3-mTagBFP-pHluorin-CAAX
(Plasmid
#178345)
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PurposeExpress a pH insensitive blue fluorencent protein mTagBFP fused to the N-terminal of a membrane targeted pH sensitive green fluorescent protein pHluorin-CAAX under CMV promoter
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 178345 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepN3
- Backbone size w/o insert (bp) 3981
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemTagBFP-pHluorin-CAAX
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SpeciesH. sapiens (human)
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Insert Size (bp)1521
- Promoter CMV
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer n/a (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pN3-mTagBFP-pHluorin-CAAX was a gift from Yulong Li (Addgene plasmid # 178345 ; http://n2t.net/addgene:178345 ; RRID:Addgene_178345) -
For your References section:
PARIS, an optogenetic method for functionally mapping gap junctions. Wu L, Dong A, Dong L, Wang SQ, Li Y. Elife. 2019 Jan 14;8. pii: 43366. doi: 10.7554/eLife.43366. 10.7554/eLife.43366 PubMed 30638447
