pDL12
(Plasmid
#17834)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 17834 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAST30
- Backbone size w/o insert (bp) 3088
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Vector typeSynthetic Biology ; Aspergillus nidulans expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namegpdA promoter, lacZ, argB,
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Alt namenutritional selection marker
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Alt nameβ-galactosidase monomer
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SpeciesA. nidulans, E. coli
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GenBank IDU24705
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer n/a
- 3′ sequencing primer M13_reverse_primer (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byFungal Genetics Stock Center of University of Kansas
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDL12 was a gift from Jay Keasling (Addgene plasmid # 17834 ; http://n2t.net/addgene:17834 ; RRID:Addgene_17834) -
For your References section:
Marker and promoter effects on heterologous expression in Aspergillus nidulans. Lubertozzi D, Keasling JD. Appl Microbiol Biotechnol. 2006 Oct . 72(5):1014-23. 10.1007/s00253-006-0368-8 PubMed 16699756