pDL11c
(Plasmid #17833)

• Depositing Lab: Jay Keasling
• Publication: Lubertozzi et al Appl Microbiol Biotechnol. 2006 Oct . 72(5):1014-23.

Backbone

• Vector backbone: pAST30
• Backbone size w/o insert (bp): 3088
• Vector type: Synthetic Biology ; Aspergillus nidulans expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: alcA promoter, lacZ, argB_1626,
• Alt name: alcohol dehydrogenase I promoter
• Alt name: β-galactosidase monomer
• Alt name: nutritional selection marker
• Species: A. nidulans, E. coli
• Mutation: The argB_1626 mutation consists of two sequential stop codons at 925 bp downstream of the start codon
• GenBank ID: U24705

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: n/a
• 3′ sequencing primer: M13_reverse_primer

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Fungal Genetics Stock Center of University of Kansas

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry