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Addgene

TB:4xHRE_RE:MiniP
(Plasmid #178313)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 178313 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUPD
  • Backbone manufacturer
    Diego Orzaez Lab
  • Backbone size w/o insert (bp) 3057
  • Total vector size (bp) 3433
  • Vector type
    Mammalian Expression, Luciferase, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    4 copies of the heat shock DNA binding motif
  • Alt name
    Transcription Blocker + 4 copies of the HRE DNA binding motif + miniP
  • Species
    H. sapiens (human), Synthetic
  • Insert Size (bp)
    376

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsmbI (destroyed during cloning)
  • 3′ cloning site BsmbI (destroyed during cloning)
  • 5′ sequencing primer gtaaaacgacggccagt
  • 3′ sequencing primer gtcatagctgtttcctg
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    TB:4xHRE_RE:MiniP was a gift from Koen Venken (Addgene plasmid # 178313 ; http://n2t.net/addgene:178313 ; RRID:Addgene_178313)

  • For your References section:

    Synthetic Assembly DNA Cloning of Multiplex Hextuple Luciferase Reporter Plasmids. Sarrion-Perdigones A, Gonzalez Y, Venken KJT. Methods Mol Biol. 2022;2524:409-432. doi: 10.1007/978-1-0716-2453-1_32. 10.1007/978-1-0716-2453-1_32 PubMed 35821490
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