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Addgene

pXCX-sGFAP-LMO-IRES-tdTomato
(Plasmid #178312)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 178312 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pXCX
  • Backbone size w/o insert (bp) 11848
  • Total vector size (bp) 13030
  • Vector type
    Mammalian Expression, Adenoviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Lactate 2-monooxygenase
  • Alt name
    LMO
  • Species
    Synthetic
  • Insert Size (bp)
    1182
  • GenBank ID
    AIU22323.1
  • Promoter sGFAP
  • Tag / Fusion Protein
    • tdTomato (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BstBI (not destroyed)
  • 3′ cloning site AgeI (not destroyed)
  • 5′ sequencing primer GFAP middle-end (ATGGGTGAGGGGAGAGCT)
  • 3′ sequencing primer IRES reverse (CCAAGTCAGTGGCTGCAC)
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pXCX-sGFAP-LMO-IRES-tdTomato was a gift from Sergey Kasparov (Addgene plasmid # 178312 ; http://n2t.net/addgene:178312 ; RRID:Addgene_178312)

  • For your References section:

    Expression of Microbial Enzymes in Mammalian Astrocytes to Modulate Lactate Release. Vaccari Cardoso B, Barrera I, Mosienko V, Gourine AV, Kasparov S, Teschemacher AG. Brain Sci. 2021 Aug 10;11(8). pii: brainsci11081056. doi: 10.3390/brainsci11081056. 10.3390/brainsci11081056 PubMed 34439675
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