pTYF-EF1a-DLDH-IRES-EGFP
(Plasmid #178310)

• Purpose: Express the bacterial D-lactate dehydrogenase (DLDH) enzyme in mammalian cells
• Depositing Lab: Sergey Kasparov
• Publication: Vaccari Cardoso et al Brain Sci. 2021 Aug 10;11(8). pii: brainsci11081056. doi: 10.3390/brainsci11081056.

Backbone

• Vector backbone: pMA-T
• Backbone size w/o insert (bp): 9791
• Total vector size (bp): 10794
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: D-lactate dehydrogenase
• Alt name: DLDH
• Species: Synthetic
• Insert Size (bp): 1002
• GenBank ID: WP013438907
• Promoter: EF1a
• Tag / Fusion Protein:
  • EGFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: EF1a-tail (GCACCTCGATTAGTTC)
• 3′ sequencing primer: GFP rev (CCGTTTACGTCGCCGTC)

Resource Information

• Supplemental Documents:
  • pTYF-EF1a-DLDH-IRES-EGFP.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry