p70rg 15
(Plasmid
#17831)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 17831 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBAD24
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Backbone manufacturerGroningen Biotechnology Center
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Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH10B
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameE. coli optimized red flourescent protein (RFPec), GFPuv
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Alt nameDsRed-express
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SpeciesDiscosoma sp., A. victoria
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GenBank IDDQ232603
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer n/a
- 3′ sequencing primer n/a (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byGFPuv -Clontech
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p70rg 15 was a gift from Jay Keasling (Addgene plasmid # 17831 ; http://n2t.net/addgene:17831 ; RRID:Addgene_17831) -
For your References section:
Combinatorial engineering of intergenic regions in operons tunes expression of multiple genes. Pfleger BF, Pitera DJ, Smolke CD, Keasling JD. Nat Biotechnol. 2006 Aug . 24(8):1027-32. 10.1038/nbt1226 PubMed 16845378