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Addgene

pCMV-LMO-IRES-EGFP
(Plasmid #178309)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 178309 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMA-T
  • Backbone size w/o insert (bp) 5081
  • Total vector size (bp) 6263
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Lactate 2-monooxygenase
  • Alt name
    LMO
  • Species
    Synthetic
  • Insert Size (bp)
    1182
  • GenBank ID
    AIU22323.1
  • Promoter CMV
  • Tag / Fusion Protein
    • EGFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (not destroyed)
  • 3′ cloning site PstI (not destroyed)
  • 5′ sequencing primer CMV forward: ATGGGCGGTAGGCGTGTA
  • 3′ sequencing primer IRES reverse: CCAAGTCAGTGGCTGCAC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-LMO-IRES-EGFP was a gift from Sergey Kasparov (Addgene plasmid # 178309 ; http://n2t.net/addgene:178309 ; RRID:Addgene_178309)

  • For your References section:

    Expression of Microbial Enzymes in Mammalian Astrocytes to Modulate Lactate Release. Vaccari Cardoso B, Barrera I, Mosienko V, Gourine AV, Kasparov S, Teschemacher AG. Brain Sci. 2021 Aug 10;11(8). pii: brainsci11081056. doi: 10.3390/brainsci11081056. 10.3390/brainsci11081056 PubMed 34439675
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