TalO8-GLC3-CFP
(Plasmid
#178307)
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PurposeCoTrIP plasmid with GLC3 promoter driving CFP's expression
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 178307 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUC
- Backbone size w/o insert (bp) 2700
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Modifications to backboneInsertion of Trp1-ARS-8xLacO
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Vector typeYeast Expression
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Selectable markersTRP1
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameGlc3pr-CFP-tAdh1
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SpeciesSynthetic
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Insert Size (bp)1400
- Promoter GLC3
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer M13
- 3′ sequencing primer M13 (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2021.10.17.464753 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
TalO8-GLC3-CFP was a gift from Brian Zid (Addgene plasmid # 178307 ; http://n2t.net/addgene:178307 ; RRID:Addgene_178307) -
For your References section:
Rvb1/Rvb2 proteins couple transcription and translation during glucose starvation. Chen YS, Hou W, Tracy S, Harvey AT, Harjono V, Xu F, Moresco JJ, Yates JR 3rd, Zid BM. Elife. 2022 Sep 15;11. pii: 76965. doi: 10.7554/eLife.76965. 10.7554/eLife.76965 PubMed 36107469