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Addgene

pUC-TalO8 (Blank-CoTrIP)
(Plasmid #178303)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 178303 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pUC
  • Backbone size (bp) 2700
  • Modifications to backbone
    Insertion of Trp1-ARS-8xLacO
  • Vector type
    Yeast Expression
  • Promoter NA
  • Selectable markers
    TRP1
  • Tag / Fusion Protein
    • NA

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Toshi Tsukiyama. Unnikrishnan et al. Nat Struct Mol Biol. 2010 Apr;17(4):430-7. doi: 10.1038/nsmb.1780. Epub 2010 Mar 14.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2021.10.17.464753 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUC-TalO8 (Blank-CoTrIP) was a gift from Brian Zid (Addgene plasmid # 178303 ; http://n2t.net/addgene:178303 ; RRID:Addgene_178303)
  • For your References section:

    Rvb1/Rvb2 proteins couple transcription and translation during glucose starvation. Chen YS, Hou W, Tracy S, Harvey AT, Harjono V, Xu F, Moresco JJ, Yates JR 3rd, Zid BM. Elife. 2022 Sep 15;11. pii: 76965. doi: 10.7554/eLife.76965. 10.7554/eLife.76965 PubMed 36107469