pAC-BETAipi-ispA/dxs
(Plasmid
#178302)
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PurposeProduces beta-carotene in E. coli; pAC-BETAipi inserted with an operon containing ispA and dxs from E. coli.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 178302 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAC-BETAipi
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Backbone manufacturerF. X. Cunningham Jr.
- Backbone size w/o insert (bp) 9259
- Total vector size (bp) 12634
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature30°C
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Growth Strain(s)Top10
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Copy numberLow Copy
Gene/Insert 1
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Gene/Insert nameispA
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SpeciesE. coli
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Insert Size (bp)900
Gene/Insert 2
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Gene/Insert namedxs
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Alt name1-deoxy-D-xylulose-5-phosphate synthase
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SpeciesE. coli
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Insert Size (bp)1863
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAC-BETAipi-ispA/dxs was a gift from Yanran Li (Addgene plasmid # 178302 ; http://n2t.net/addgene:178302 ; RRID:Addgene_178302) -
For your References section:
Establishment of strigolactone-producing bacterium-yeast consortium. Wu S, Ma X, Zhou A, Valenzuela A, Zhou K, Li Y. Sci Adv. 2021 Sep 17;7(38):eabh4048. doi: 10.1126/sciadv.abh4048. Epub 2021 Sep 17. 10.1126/sciadv.abh4048 PubMed 34533983