pAG414GPD-SlCYP722C(pYL1071)
(Plasmid
#178294)
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PurposeExpresses SlCYP722C from S. lycopersicum in Saccharomyces cerevisiae. Centromeric TRP, PGPD-SlCYP722C-TCYC1
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 178294 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepAG414GPD-ccdB
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Backbone manufacturerSusan Lindquist
- Backbone size w/o insert (bp) 7387
- Total vector size (bp) 7220
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Vector typeYeast Expression
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Selectable markersTRP1
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameSlCYP722C
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Alt nameSlCYP722C from S. lycopersicum
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SpeciesSolanum lycopersicum
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Insert Size (bp)1461
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GenBank IDXP_004232430.1
- Promoter GAP
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer M13 rev
- 3′ sequencing primer M13 for (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAG414GPD-SlCYP722C(pYL1071) was a gift from Yanran Li (Addgene plasmid # 178294 ; http://n2t.net/addgene:178294 ; RRID:Addgene_178294) -
For your References section:
Establishment of strigolactone-producing bacterium-yeast consortium. Wu S, Ma X, Zhou A, Valenzuela A, Zhou K, Li Y. Sci Adv. 2021 Sep 17;7(38):eabh4048. doi: 10.1126/sciadv.abh4048. Epub 2021 Sep 17. 10.1126/sciadv.abh4048 PubMed 34533983
Map uploaded by the depositor.
