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Addgene

pET21a-trAtCCD8(pYL735)
(Plasmid #178286)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 178286 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET21a
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5443
  • Total vector size (bp) 6930
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    trAtCCD8
  • Alt name
    N-terminus 56 amino-acid truncated CCD8 from Arabidopsis
  • Species
    A. thaliana
  • Insert Size (bp)
    1548
  • GenBank ID
    NP_195007.2
  • Promoter T7

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer T7 primer
  • 3′ sequencing primer T7 terminator primer
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET21a-trAtCCD8(pYL735) was a gift from Yanran Li (Addgene plasmid # 178286 ; http://n2t.net/addgene:178286 ; RRID:Addgene_178286)

  • For your References section:

    Establishment of strigolactone-producing bacterium-yeast consortium. Wu S, Ma X, Zhou A, Valenzuela A, Zhou K, Li Y. Sci Adv. 2021 Sep 17;7(38):eabh4048. doi: 10.1126/sciadv.abh4048. Epub 2021 Sep 17. 10.1126/sciadv.abh4048 PubMed 34533983
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