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pCDFDuet-trAtCCD7-OsD27(pYL726 )
(Plasmid #178285)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 178285 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDFDuet-1
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 3781
  • Total vector size (bp) 6189
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    OsD27
  • Alt name
    D27 from Oryza sativa
  • Species
    Oryza sativa
  • Insert Size (bp)
    837
  • GenBank ID
    QSV51849.1
  • Promoter T7

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site Nde I (not destroyed)
  • 3′ cloning site Avr II (not destroyed)
  • 5′ sequencing primer DuetUP2 Primer
  • 3′ sequencing primer T7 Terminator Primer
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    trAtCCD7
  • Alt name
    N-terminus 31 amino-acid truncated CCD7 from Arabidopsis
  • Species
    A. thaliana
  • Insert Size (bp)
    1782
  • GenBank ID
    NP_001324720.1
  • Promoter T7

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site Nco I (not destroyed)
  • 3′ cloning site Not I (not destroyed)
  • 5′ sequencing primer ACYCDuetUP1
  • 3′ sequencing primer DuetDOWN1
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDFDuet-trAtCCD7-OsD27(pYL726 ) was a gift from Yanran Li (Addgene plasmid # 178285 ; http://n2t.net/addgene:178285 ; RRID:Addgene_178285)

  • For your References section:

    Establishment of strigolactone-producing bacterium-yeast consortium. Wu S, Ma X, Zhou A, Valenzuela A, Zhou K, Li Y. Sci Adv. 2021 Sep 17;7(38):eabh4048. doi: 10.1126/sciadv.abh4048. Epub 2021 Sep 17. 10.1126/sciadv.abh4048 PubMed 34533983
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