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Addgene

pBAD*RFPEC2
(Plasmid #17823)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 17823 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pBAD24
  • Backbone manufacturer
    Groningen Biotechnology Center
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH10B
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    E. coli optimized red flourescent protein (RFPec2)
  • Alt name
    DsRed-express
  • Species
    Discosoma sp.
  • GenBank ID
    AJ851288

Cloning Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that this plasmid contains GFP as well as RFP.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBAD*RFPEC2 was a gift from Jay Keasling (Addgene plasmid # 17823 ; http://n2t.net/addgene:17823 ; RRID:Addgene_17823)
  • For your References section:

    Optimization of DsRed production in Escherichia coli: effect of ribosome binding site sequestration on translation efficiency. Pfleger BF, Fawzi NJ, Keasling JD. Biotechnol Bioeng. 2005 Dec 5. 92(5):553-8. 10.1002/bit.20630 PubMed 16247774