pXint129
(Plasmid #178208)

• Purpose: Encodes bacteriophage lamda excisionase and integrase to facilitate in vivo Gateway LR clonase reaction.
• Depositing Lab: Gregory Phillips
• Publication: Platt et al Plasmid. 2000 Jan . 43(1):12-23.

Backbone

• Vector backbone: pSC101, pPS2-3DRS, pSPORT2, pLDR8
• Backbone size w/o insert (bp): 6100
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: xis excisionase, int integrase
• Alt name: λxis
• Alt name: λint
• Species: Bacteriophage lambda
• Insert Size (bp): 2500
• GenBank ID: 2703504 2703464
• Entrez Gene: xis (a.k.a. lambdap34)
• Entrez Gene: lambdap32 (a.k.a. lambdap32)
• Promoter: lacI

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: unknown

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Induction of xis and int is controlled by lactose promoter.