rASAP
(Plasmid
#178205)
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PurposeRatiometric version of the genetically encoded voltage indicator ASAP2s-I67T-Q397R.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 178205 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCDNA3.1(+)
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namerASAP
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SpeciesG. gallus (chicken); E. quadricolor
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Insert Size (bp)1992
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
rASAP was a gift from Stefan Heinemann (Addgene plasmid # 178205 ; http://n2t.net/addgene:178205 ; RRID:Addgene_178205) -
For your References section:
Monitoring of compound resting membrane potentials of cell cultures with ratiometric genetically encoded voltage indicators. Ruhl P, Langner JM, Reidel J, Schonherr R, Hoshi T, Heinemann SH. Commun Biol. 2021 Oct 7;4(1):1164. doi: 10.1038/s42003-021-02675-0. 10.1038/s42003-021-02675-0 PubMed 34620975
