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Addgene

pBMCL1
(Plasmid #178203)

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Item Catalog # Description Quantity Price (USD)
Plasmid 178203 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pFastBac1
  • Backbone manufacturer
    Thermofisher
  • Backbone size (bp) 4775
  • Modifications to backbone
    adding multiple elements for protein expression in mammalian cells
  • Vector type
    Mammalian Expression
  • Promoter CMV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ sequencing primer ggtcttactgacatc
  • 3′ sequencing primer ccagtcaatctttcac
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBMCL1 was a gift from Lei Chen (Addgene plasmid # 178203 ; http://n2t.net/addgene:178203 ; RRID:Addgene_178203)

  • For your References section:

    A co-expression vector for baculovirus-mediated protein expression in mammalian cells. Guo W, Wang M, Chen L. Biochem Biophys Res Commun. 2022 Feb 26;594:69-73. doi: 10.1016/j.bbrc.2022.01.056. Epub 2022 Jan 19. 10.1016/j.bbrc.2022.01.056 PubMed 35074588
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