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Addgene

pRD424
(Plasmid #178202)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 178202 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC19
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    phns + rok
  • Species
    Escherichia coli (promoter) + Bacillus subtilis (coding region)
  • Promoter hns promoter

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRD424 was a gift from Remus Dame (Addgene plasmid # 178202 ; http://n2t.net/addgene:178202 ; RRID:Addgene_178202)

  • For your References section:

    The B. subtilis Rok protein is an atypical H-NS-like protein irresponsive to physico-chemical cues. Erkelens AM, Qin L, van Erp B, Miguel-Arribas A, Abia D, Keek HGJ, Markus D, Cajili MKM, Schwab S, Meijer WJJ, Dame RT. Nucleic Acids Res. 2022 Nov 21. pii: 6835367. doi: 10.1093/nar/gkac1064. 10.1093/nar/gkac1064 PubMed 36408910
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