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Addgene

pCI neo-hEST2-HA
(Plasmid #1782)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 1782 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCI neo
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    hTERT
  • Alt name
    Ever Shorter Telomeres 2
  • Alt name
    telomerase reverse transcriptase
  • Alt name
    hTERT-HA
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3510
  • Mutation
    Clone is catalytically active but is dead in vivo.
  • GenBank ID
    AF018167
  • Entrez Gene
    TERT (a.k.a. CMM9, DKCA2, DKCB4, EST2, PFBMFT1, TCS1, TP2, TRT, hEST2, hTRT)
  • Tag / Fusion Protein
    • HA (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer EBV-rev
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCI neo-hEST2-HA was a gift from Bob Weinberg (Addgene plasmid # 1782 ; http://n2t.net/addgene:1782 ; RRID:Addgene_1782)

  • For your References section:

    hEST2, the putative human telomerase catalytic subunit gene, is up-regulated in tumor cells and during immortalization. Meyerson M, Counter CM, Eaton EN, Ellisen LW, Steiner P, Caddle SD, Ziaugra L, Beijersbergen RL, Davidoff MJ, Liu Q, Bacchetti S, Haber DA, Weinberg RA. Cell 1997 Aug 22;90(4):785-95. 10.1016/S0092-8674(00)80538-3 PubMed 9288757
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