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Addgene

pRD461
(Plasmid #178198)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 178198 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET30b
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    rok 6xHis
  • Species
    Bacillus subtilis
  • Promoter T7
  • Tag / Fusion Protein
    • His-tag (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRD461 was a gift from Remus Dame (Addgene plasmid # 178198 ; http://n2t.net/addgene:178198 ; RRID:Addgene_178198)

  • For your References section:

    The B. subtilis Rok protein is an atypical H-NS-like protein irresponsive to physico-chemical cues. Erkelens AM, Qin L, van Erp B, Miguel-Arribas A, Abia D, Keek HGJ, Markus D, Cajili MKM, Schwab S, Meijer WJJ, Dame RT. Nucleic Acids Res. 2022 Nov 21. pii: 6835367. doi: 10.1093/nar/gkac1064. 10.1093/nar/gkac1064 PubMed 36408910
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