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Addgene

pRD415
(Plasmid #178197)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 178197 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET30b
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    rok _linker
  • Species
    Bacillus subtilis
  • Mutation
    deletion of residues 75-96
  • Promoter T7

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRD415 was a gift from Remus Dame (Addgene plasmid # 178197 ; http://n2t.net/addgene:178197 ; RRID:Addgene_178197)

  • For your References section:

    The B. subtilis Rok protein is an atypical H-NS-like protein irresponsive to physico-chemical cues. Erkelens AM, Qin L, van Erp B, Miguel-Arribas A, Abia D, Keek HGJ, Markus D, Cajili MKM, Schwab S, Meijer WJJ, Dame RT. Nucleic Acids Res. 2022 Nov 21. pii: 6835367. doi: 10.1093/nar/gkac1064. 10.1093/nar/gkac1064 PubMed 36408910
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