pRD415
(Plasmid
#178197)
-
PurposeRok _Linker overexpression
-
Depositing Lab
-
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 178197 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepET30b
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert namerok _linker
-
SpeciesBacillus subtilis
-
Mutationdeletion of residues 75-96
- Promoter T7
Cloning Information
- Cloning method Gibson Cloning
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pRD415 was a gift from Remus Dame (Addgene plasmid # 178197 ; http://n2t.net/addgene:178197 ; RRID:Addgene_178197) -
For your References section:
The B. subtilis Rok protein is an atypical H-NS-like protein irresponsive to physico-chemical cues. Erkelens AM, Qin L, van Erp B, Miguel-Arribas A, Abia D, Keek HGJ, Markus D, Cajili MKM, Schwab S, Meijer WJJ, Dame RT. Nucleic Acids Res. 2022 Nov 21. pii: 6835367. doi: 10.1093/nar/gkac1064. 10.1093/nar/gkac1064 PubMed 36408910
