pEF274 SVAfw
(Plasmid #178192)

• Purpose: The SVA (SINE-VNTR-ALU) retroposon that causes human X-linked Dystonia Parkinsonism (XDP), within a BAC (pJazz) backbone.
• Depositing Lab: Elizabeth Fisher
• Publication: SVA sequence that is causative for human X-lnked dystonia Parkinsonism (XDP) (unpublished)

Backbone

• Vector backbone: pJazz
• Backbone manufacturer: Lucigen.com
• Backbone size w/o insert (bp): 12000
• Total vector size (bp): 15400
• Vector type: Linear BAC vector

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Growth instructions: Slower growing conditions (liquid culture: 30°C, 24 h, 150 rpm; plates: 30°C, 36 h; 12.5 μg/ml chloramphenicol) are necessary for proper maintenance of repeat sequences.
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: SVA that inserts into the X-linked human TAF1 gene to cause X-linked dystonia Parkinsonism
• Species: H. sapiens (human)
• Insert Size (bp): 3400

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Dra 1 (unknown if destroyed)
• 3′ cloning site: Dra 1 (unknown if destroyed)
• 5′ sequencing primer: AAATGGTCAGTTAATCAGTTCT- in pJAZZ backbone
• 3′ sequencing primer: CAGTCCAGTTACGCTGGAGTC- in pJAZZ backbone

Resource Information

• Supplemental Documents:
  • Annotated Depositor's Sequence
• A portion of this plasmid was derived from a plasmid made by: Cris Bragg, Mass General Hospital, Boston () provided the insert in a topo vector, we cloned it into a pJazz linear BAC vector.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This clone contains the SVA (SINE-VNTR-ALU repeat) that when inserted into intron 32 of the human TAF1 gene causes X-linked dystonia Parkinsonism (XDP). Importantly, the SVA is cloned into a linear BAC vector which allows the 53 consecutive hexanucleotide repeats of this SVA to stay stable when propagated at 30oC i.e. no repeat expansions or reductions. SVA originally came from the lab of Dr. Cris Bragg, MGH, Boston, USA.

Please Note: Addgene NGS found some T nucleotides present within the poly-A tail. This is not known to affect the plasmid's function.