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Addgene

pDEST-HisMBP-SED1
(Plasmid #178191)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 178191 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDEST
  • Backbone size w/o insert (bp) 5490
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SED1 biosensor
  • Alt name
    His-MBP-mCerulean3-AtLEA4-5-Citrine
  • Species
    Synthetic
  • Insert Size (bp)
    3111
  • Promoter tac
  • Tag / Fusion Protein
    • 6xHis-MBP (N terminal on insert)

Cloning Information

  • Cloning method Gateway Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDEST-HisMBP-SED1 was a gift from Cesar Cuevas-Velazquez & Jose Dinneny (Addgene plasmid # 178191 ; http://n2t.net/addgene:178191 ; RRID:Addgene_178191)

  • For your References section:

    Intrinsically disordered protein biosensor tracks the physical-chemical effects of osmotic stress on cells. Cuevas-Velazquez CL, Vellosillo T, Guadalupe K, Schmidt HB, Yu F, Moses D, Brophy JAN, Cosio-Acosta D, Das A, Wang L, Jones AM, Covarrubias AA, Sukenik S, Dinneny JR. Nat Commun. 2021 Sep 14;12(1):5438. doi: 10.1038/s41467-021-25736-8. 10.1038/s41467-021-25736-8 PubMed 34521831
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