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pGL3-mBcl11b-MAR4
(Plasmid #178188)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 178188 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGL3-promoter
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 5010
  • Total vector size (bp) 5405
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Bcl11b MAR4
  • Alt name
    Ctip2 MAR4
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    420
  • GenBank ID
    NM_001286343.1
  • Promoter SV40 promoter

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer RVprimer3 CTAGCAAAATAGGCTGTCCC
  • 3′ sequencing primer GLprimer2 CTTTATGTTTTTGGCGTCTTCCA
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGL3-mBcl11b-MAR4 was a gift from Katsuhiko Ono (Addgene plasmid # 178188 ; http://n2t.net/addgene:178188 ; RRID:Addgene_178188)

  • For your References section:

    Species-Specific Mechanisms of Neuron Subtype Specification Reveal Evolutionary Plasticity of Amniote Brain Development. Nomura T, Yamashita W, Gotoh H, Ono K. Cell Rep. 2018 Mar 20;22(12):3142-3151. doi: 10.1016/j.celrep.2018.02.086. 10.1016/j.celrep.2018.02.086 PubMed 29562171
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