modRNAc0-ABE8e-P2A-GFP
(Plasmid #178177)

• Purpose: IVT template plasmid for making ABE8e-P2A-GFP modRNA
• Depositing Lab: Xiaojun Lian
• Publication: Haideri et al Cell Rep Methods. 2022 Sep 7;2(9):100290. doi: 10.1016/j.crmeth.2022.100290. eCollection 2022 Sep 19.

Backbone

• Vector backbone: modRNA cap0 plasmid
• Vector type: IVT template plasmid

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ABE8e
• Species: Synthetic; base editor ABE8e
• GenBank ID:
• Promoter: T7 promoter
• Tag / Fusion Protein:
  • P2A-GFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoR1 (not destroyed)
• 3′ cloning site: Spe1 (not destroyed)
• 5′ sequencing primer: T7 promoter
• 3′ sequencing primer: M13 Rvs

Resource Information

• A portion of this plasmid was derived from a plasmid made by: ABE8e was cloned from addgene plasmid 138495 ABE8e(TadA-8e V106W).
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/2022.05.24.493220v1 for bioRxiv preprint.