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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 17817 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonen/a
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Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Tetracycline, 10 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH10B
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namemevalonate kinase, phosphomevalonate kinase, mevalonate pyrophosphate decarboxylatse, IPP isomerase, FPP synthase
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SpeciesS. cerevisiae (budding yeast); E. coli
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer n/a
- 3′ sequencing primer n/a (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
does not contain lacI/
ERG1, ERG8, MVD1: S. cerevisiae/
idi, ispA: E. Coli
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMBIS was a gift from Jay Keasling (Addgene plasmid # 17817 ; http://n2t.net/addgene:17817 ; RRID:Addgene_17817) -
For your References section:
Engineering a mevalonate pathway in Escherichia coli for production of terpenoids. Martin VJ, Pitera DJ, Withers ST, Newman JD, Keasling JD. Nat Biotechnol. 2003 Jul . 21(7):796-802. 10.1038/nbt833 PubMed 12778056
