pPro24(s)-gfp
(Plasmid
#17813)
-
Depositing Lab
-
Publication
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 17813 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonen/a
-
Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberLow Copy
Gene/Insert
-
Gene/Insert nameGFPuv
-
SpeciesA. victoria
-
Insert Size (bp)712
-
MutationD234N compared to AF007834
-
GenBank IDAF007834
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer n/a
- 3′ sequencing primer pBAD Reverse (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Provided in strain JSB, a slightly modified version of E. coli strain BL21 with genotype: BL21(DE3)/D(sbm-ygfDGHI).
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pPro24(s)-gfp was a gift from Jay Keasling (Addgene plasmid # 17813 ; http://n2t.net/addgene:17813 ; RRID:Addgene_17813) -
For your References section:
Propionate-regulated high-yield protein production in Escherichia coli. Lee SK, Keasling JD. Biotechnol Bioeng. 2006 Apr 5. 93(5):912-8. 10.1002/bit.20784 PubMed 16333863