pT7-hMLH1dn for IVT
(Plasmid #178114)

• Purpose: Template for in vitro transcription of human MLH1dn
• Depositing Lab: David Liu
• Publication: Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29. doi: 10.1016/j.cell.2021.09.018.

Backbone

• Vector backbone: pT7
• Modifications to backbone: Inactivated T7 promoter to prevent transcription from plasmid
• Vector type: Template for in vitro transcription

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: human MLH1dn (codon optimized)
• Alt name: homo sapiens MLH1 del754-756
• Species: H. sapiens (human)
• Insert Size (bp): 2259
• Mutation: Detailed in manuscript
• Entrez Gene: MLH1 (a.k.a. COCA2, FCC2, HNPCC, HNPCC2, LYNCH2, MLH-1, MMRCS1, hMLH1)
• Promoter: T7 (inactivated)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: CMV-forward
• 3′ sequencing primer: BGH-reverse

Resource Information

• Supplemental Documents:
  • hMLH1dn for IVT.gb
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry