pPro33
(Plasmid #17810)

• Purpose: (Empty Backbone)
• Depositing Lab: Jay Keasling
• Publication: Lee et al Appl Environ Microbiol. 2005 Nov . 71(11):6856-62.

Backbone

• Vector backbone: pPro33
• Backbone size (bp): 5878
• Vector type: Bacterial Expression, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH10B
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: None

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: n/a
• 3′ sequencing primer: pBAD reverse

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

After deletion of the NheI site in the front of pACYC origin region of pBAD33 by partial digestion with NheI, T4 DNA polymerase treatment, and self-ligation, the ClaI-NheI prpR-PprpB region from pPro18 was ligated to the large fragments of pBAD33 resulting from digestion with the same enzymes, creating pPro33.