pCI neo-hEST2
(Plasmid #1781)

• Depositing Lab: Bob Weinberg
• Publication: Meyerson et al Cell 1997 Aug 22;90(4):785-95.

Backbone

• Vector backbone: pCI neo
• Backbone size w/o insert (bp): 5400
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: hTERT
• Alt name: Ever Shorter Telomeres 2
• Alt name: telomerase reverse transcriptase
• Alt name: pCI-hTERT
• Species: H. sapiens (human)
• Insert Size (bp): 3450
• GenBank ID: AF018167
• Entrez Gene: TERT (a.k.a. CMM9, DKCA2, DKCB4, EST2, PFBMFT1, TCS1, TP2, TRT, hEST2, hTRT)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: EBV-rev

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry