pMARO
(Plasmid #178086)

• Purpose: Derivative of pMK2010 that contains tetracycline resistance insert between attL1 and attL2 flanked by EcorV. Suitable as control or backbone for library construction for in vivo Gateway reaction.
• Depositing Lab: Sören Abel
• Publication: Gillman et al Plasmid. 2021 Nov;118:102608. doi: 10.1016/j.plasmid.2021.102608. Epub 2021 Nov 18.

Backbone

• Vector backbone: pMK2010
• Backbone size w/o insert (bp): 2677
• Total vector size (bp): 4621
• Modifications to backbone: Introduced unique EcorV restriction sites to flank the tetracycline resistance cassette that allow removal and digestion of the tetA/tetR for library construction.
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Growth with tetracycline and kanamycin to maintain tetA and tetR inserts for use as a control for in vivo Gateway is recommended.
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: TcR (tetA/tetR)
• Alt name: Tetracycline Resistant Cassette
• Species: Escherichia coli
• Insert Size (bp): 1944
• Mutation: Tetracycline resistance cassette is flanked by EcorV sites
• GenBank ID: ABO32180.1 ABO32181.1

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: tggcagttccctactctcg
• 3′ sequencing primer: tttgagacacgggccagag

Resource Information

• Supplemental Documents:
  • pMARO.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The plasmids from this article are also used with plasmid pXint129 (www.addgene.org/178208)