pANG03
(Plasmid #178085)

• Purpose: (Empty Backbone) Tetracycline resistant backbone for in vivo Gateway reaction. Plasmid contains gateway cassette with restriction sites to add promoters or C and N Terminal tags. Compatible with pANG02.
• Depositing Lab: Sören Abel
• Publication: Gillman et al Plasmid. 2021 Nov;118:102608. doi: 10.1016/j.plasmid.2021.102608. Epub 2021 Nov 18.

Backbone

• Vector backbone: pANG01, PRK415
• Backbone manufacturer: NA, NA
• Backbone size (bp): 2971
• Modifications to backbone: pANG03 was constructed by replacing the bla resistance gene from pANG01 with tetAR from pRK415.
• Vector type: Bacterial Expression
• Promoter: NA

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Tetracycline, 25 & 10 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): ccdB Survival
• Growth instructions: Plasmid contains ccdB toxin. Strain grows under standard laboratory conditions in LB media supplemented with antibiotics to maintain plasmid.
• Copy number: Low Copy

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: M13 Forward for Gateway site
• 3′ sequencing primer: M13 Reverse for Gateway site

Resource Information

• Supplemental Documents:
  • pANG03.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The plasmids from this article are also used with plasmid pXint129 (www.addgene.org/178208)