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Addgene

pANG01
(Plasmid #178083)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 178083 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGP704, pBAD33, pDONR201
  • Backbone manufacturer
    NA, NA, Invitrogen
  • Modifications to backbone
    pANG01 was constructed with the bla from pGP704, oriTRP4 from pG704, and the oriVp15a from pBAD33. The Gateway sequence was obtained from commercial vector pDONR201 (Invitrogen) and inserted into the EcorV sites of minimal vectors by Gibson assembly to include unique PmeI and PacI restriction sites flanking the att sites.
  • Vector type
    Bacterial Expression
  • Promoter NA

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin, 25 & 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Growth instructions
    Plasmid contains ccdB toxin. Strain grows under standard laboratory conditions in LB media supplemented with antibiotics to maintain plasmid.
  • Copy number
    Low Copy

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer M13 Forward for Gateway site
  • 3′ sequencing primer M13 Reverse for Gateway site
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The plasmids from this article are also used with plasmid pXint129 (www.addgene.org/178208)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pANG01 was a gift from Sören Abel (Addgene plasmid # 178083 ; http://n2t.net/addgene:178083 ; RRID:Addgene_178083)

  • For your References section:

    A single step three-strain in vivo Gateway reaction. Gillman AN, Helleux A, Abel S. Plasmid. 2021 Nov;118:102608. doi: 10.1016/j.plasmid.2021.102608. Epub 2021 Nov 18. 10.1016/j.plasmid.2021.102608 PubMed 34801582
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