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pACEBac1-gamma-TuSC
(Plasmid #178079)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 178079 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pACEBac1
  • Backbone manufacturer
    Geneva Biotech
  • Total vector size (bp) 10949
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Gentamicin, 10 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    gamma-tubulin
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1353
  • Entrez Gene
    TUBG1 (a.k.a. CDCBM4, GCP-1, TUBG, TUBGCP1)
  • Tag / Fusion Protein
    • TEV-His6 (C terminal on insert)

Gene/Insert 2

  • Gene/Insert name
    GCP2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2790
  • Entrez Gene
    TUBGCP2 (a.k.a. ALP4, GCP-2, GCP2, Grip103, PAMDDFS, SPBC97, SPC97, Spc97p, h103p, hGCP2, hSpc97)

Gene/Insert 3

  • Gene/Insert name
    GCP3
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2721
  • Entrez Gene
    TUBGCP3 (a.k.a. 104p, ALP6, GCP3, Grip104, SPBC98, Spc98, Spc98p)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Assembled via BstXI/Iceu-I restriction digestion following the MultiBac manual

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pACEBac1-gamma-TuSC was a gift from Tarun Kapoor (Addgene plasmid # 178079 ; http://n2t.net/addgene:178079 ; RRID:Addgene_178079)

  • For your References section:

    Biochemical reconstitutions reveal principles of human gamma-TuRC assembly and function. Wieczorek M, Ti SC, Urnavicius L, Molloy KR, Aher A, Chait BT, Kapoor TM. J Cell Biol. 2021 Mar 1;220(3). pii: 211719. doi: 10.1083/jcb.202009146. 10.1083/jcb.202009146 PubMed 33496729
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