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pACEBac1-gamma-tubulin(N229A)-TEV-His6
(Plasmid #178077)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 178077 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pACEBac1
  • Backbone manufacturer
    Geneva Biotech
  • Total vector size (bp) 4234
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Gentamicin, 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    gamma-tubulin with an N229A point mutation
  • Alt name
    TUBG1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1353
  • Mutation
    Changed Asparagine 229 to Alanine
  • Entrez Gene
    TUBG1 (a.k.a. CDCBM4, GCP-1, TUBG, TUBGCP1)
  • Tag / Fusion Protein
    • TEV-His6 (C terminal on insert)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Originally published in Murphy et al., 2001 and received from Dr. Jens Luders (Institute for Research in Biomedicine, Barcelona, Spain)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Primers used to generate pACEBac1-gamma-tubulin(N229A)-TEV-His6 via site-directed mutagenesis were CCCATCCTTCTCCCAGATCGCCCAGCTGGTGTCTACCATCATGTC and GACATGATGGTAGACACCAGCTGGGCGATCTGGGAGAAGGATGGG

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pACEBac1-gamma-tubulin(N229A)-TEV-His6 was a gift from Tarun Kapoor (Addgene plasmid # 178077 ; http://n2t.net/addgene:178077 ; RRID:Addgene_178077)

  • For your References section:

    Biochemical reconstitutions reveal principles of human gamma-TuRC assembly and function. Wieczorek M, Ti SC, Urnavicius L, Molloy KR, Aher A, Chait BT, Kapoor TM. J Cell Biol. 2021 Mar 1;220(3). pii: 211719. doi: 10.1083/jcb.202009146. 10.1083/jcb.202009146 PubMed 33496729
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