pMW96
(Plasmid
#178066)
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PurposeExpression of His6-SUMO-TEV-GFP-PreScission-γ-TuNA in E. coli
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 178066 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET His6 Sumo TEV LIC cloning vector (1S), gift from Scott Gardia
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Backbone manufacturerAddgene plasmid # 29659
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameCDK5RAP2
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Alt nameCEP215
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SpeciesH. sapiens (human)
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Insert Size (bp)150
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Entrez GeneCDK5RAP2 (a.k.a. C48, Cep215, MCPH3)
Resource Information
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A portion of this plasmid was derived from a plasmid made bypRcCMV Cep215 (Nigg CW493) Addgene plasmid # 41152; http://n2t.net/addgene:41152; RRID:Addgene_41152
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMW96 was a gift from Tarun Kapoor (Addgene plasmid # 178066 ; http://n2t.net/addgene:178066 ; RRID:Addgene_178066) -
For your References section:
Asymmetric Molecular Architecture of the Human gamma-Tubulin Ring Complex. Wieczorek M, Urnavicius L, Ti SC, Molloy KR, Chait BT, Kapoor TM. Cell. 2020 Jan 9;180(1):165-175.e16. doi: 10.1016/j.cell.2019.12.007. Epub 2019 Dec 17. 10.1016/j.cell.2019.12.007 PubMed 31862189