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Addgene

pcDNA3-Y478C-WLS-HA-IRES-GFP
(Plasmid #178063)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 178063 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Addgene (#51406)
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    WLS
  • Species
    H. sapiens (human)
  • Mutation
    Y478C
  • Entrez Gene
    WLS (a.k.a. C1orf139, EVI, GPR177, MRP, mig-14)
  • Tags / Fusion Proteins
    • HA (C terminal on insert)
    • IRES-GFP (C terminal on backbone)

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-Y478C-WLS-HA-IRES-GFP was a gift from Joseph Gleeson (Addgene plasmid # 178063 ; http://n2t.net/addgene:178063 ; RRID:Addgene_178063)

  • For your References section:

    A Human Pleiotropic Multiorgan Condition Caused by Deficient Wnt Secretion. Chai G, Szenker-Ravi E, Chung C, Li Z, Wang L, Khatoo M, Marshall T, Jiang N, Yang X, McEvoy-Venneri J, Stanley V, Anzenberg P, Lang N, Wazny V, Yu J, Virshup DM, Nygaard R, Mancia F, Merdzanic R, Toralles MBP, Pitanga PML, Puri RD, Hernan R, Chung WK, Bertoli-Avella AM, Al-Sannaa N, Zaki MS, Willert K, Reversade B, Gleeson JG. N Engl J Med. 2021 Sep 30;385(14):1292-1301. doi: 10.1056/NEJMoa2033911. 10.1056/NEJMoa2033911 PubMed 34587386
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