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pIRESneo2 mycBioID-Tollip M240A,F241A
(Plasmid #178055)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 178055 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pIRESneo2
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 5300
  • Total vector size (bp) 7140
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Toll-Interacting Protein
  • Alt name
    IL-1RAcPIP
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1840
  • Mutation
    Changed Methionine 240 to Alanine and changed Phenylalanine 241 to Alanine.
  • GenBank ID
    NM_019009.4
  • Entrez Gene
    TOLLIP (a.k.a. IL-1RAcPIP)
  • Promoter CMV
  • Tag / Fusion Protein
    • mycBioID (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer CMV FWD
  • 3′ sequencing primer IRES REV
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pIRESneo2 mycBioID-Tollip M240A,F241A was a gift from David Tumbarello (Addgene plasmid # 178055 ; http://n2t.net/addgene:178055 ; RRID:Addgene_178055)

  • For your References section:

    Tollip coordinates Parkin-dependent trafficking of mitochondrial-derived vesicles. Ryan TA, Phillips EO, Collier CL, Jb Robinson A, Routledge D, Wood RE, Assar EA, Tumbarello DA. EMBO J. 2020 Jun 2;39(11):e102539. doi: 10.15252/embj.2019102539. Epub 2020 Apr 20. 10.15252/embj.2019102539 PubMed 32311122
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